Whole+Slide+High+Resolution+Scanners

I suspect that almost everyone has seen Google Earth and images from the Hubble Telescope. You start with a very low power image of earth, and progressively zoom in at every higher magnification and //**HIgher Resolution**//.
 * HIGH RESOLUTION WHOLE SLIDE SCANNING**

Traditional images of complete slides of brains of even relatively small brained animals, such as mice or chicks or goldfish, are taken at low magnification and low resolution. As you zoom in on such images, the pictures becomes ever more blurry. In order to view details of the slide, it was necessary to collect higher magnification images, at great time and expense. Matching the resulting images to the original low power view was often difficult.

Visit http://www.brainmaps.org, You will find many complete series of images of monkeys, platypus, rats, mice, chicks, goldfish, and other animals. As you zoom in on an image, the detail provided is comparable to that which you might see with a 20X lens with high numerical aperture (n.a. or N.A.) and high resolution, contrast and brightness. The impact of these images upon an experienced microscopist is nothing short of astonishing. The image remains centered, sharp and crisp. You can easily recognize single cells, axons, dendrites, contours, blood vessels, glia, etc. If you open another window on your browser, you can look at both a low power view of the same section, while viewing the high magnification image at the same time. A navigation window helps you maintain your orientation within the section.

Equally impressive is that you can instantly step from one section to the next. If the series of images were aligned to even approximate their original position, you can step through the brains with speed, and follow the changing shape and content of individual subnuclei.

This technology allows microscopists to rapidly review a sequence of slides from the same animal, or brains of different animals, in different planes or with varying immunohistochemical properties.

You can share images and experiments with colleagues in other parts of the world.

What is the technology underlying this new type of microscopy?

This WIKI website will describe the technology underlying these images, and will help you learn how to prepare, access, use and share similar digital image files of your favorite slides.

1) Whole slide scanning 2) Tissue Preparation 3) Scanners 4) Resolution 5) Image storage 6) Image retrieval and review 7) Image distribution over the internet 8) Image analysis

A major landmark was recently established with the adoption of Digital Imaging and Communication in Medicine (DICOM) Supplement 145, establishing the first accepted standards for WSI (Whole Slide Imaging) / WFHR (Wide Field High Resolution).

The file provides a useful explanation of the file format currently employed by Aperio, Nanozoomer, and other manufacturers of WSI instruments.